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Officials said Indiana State Trooper Edward Titus and Grant County Deputy Joshua Kennedy entered the trailer and were able to rescue the animals. The pair along with a worker for the Shrine Circus in Fort Wayne, Ind., rescued five zebras, four camels and a miniature horse from the trailer.
"Additional officers on the scene were able to secure all the animals until other trucks rom the Shrine Circus arrived," the sheriff's department said.
No animals were injured or missing, but Titus and Kennedy were treated for smoke inhalation.
The preliminary crash investigation by State Police revealed that an equipment failure was the cause of the fire.
"Grateful to report that all animals involved have been rescued safely. No harm to our furry friends," the Grant County Sheriff's Department posted on Facebook.
The truck fire kept the interstate's northbound lanes closed until 6:30 a.m.