"I had never had such a wonderful creature. It was harder losing him than any other cat I've ever had," the woman told The Courier newspaper.
Living tissue samples were collected by Dr. Kevin Christman of Cedar Valley Veterinary Center in Cedar Falls and sent along to ViaGen Pets in Cedar Park, Texas.
ViaGen Pets specializes in genetic preservation and cloning for household pets. The firm has cloned dogs, cats and horses in the past and recently made headlines for cloning an endangered Przewalski's horse for San Diego Zoo.
ViaGen preserved the samples until after Mr. Tufts' death, when his owner requested the cloning go forward.
Melain Rodriguez, ViaGen client services manager, said specialists used one of the frozen cells to replace the nucleus of a female cat's egg. The embryo was then transferred to a surrogate mother cat in a method similar to in vitro fertilization.
Mr. Tufts' genetic twin, Mr. Tufts Jr., is now 9 months old. He came to live with his owner, who also adopted the surrogate mother cat, when he was 2 months old.
"The only physical difference, as far as I can see, is in health and body condition. The original T had been found on a forest trail and had a very bad respiratory illness," the owner said.
ViaGen said cloned pets maintain the same appearance, intelligence and temperament as the animals they are cloned from.
"When we produce the clone it's an identical twin. There is zero genetic modification occurring," Blake Russell, president of ViaGen Pets, told KXAN-TV.
The feline's owner said Mr. Tufts Jr. reminds her a lot of his namesake, although she has noticed some differences.
"Our new Mr. Tufts Jr. is much more athletic than our original, probably because he, and mom cat, too, had the best of care," she said.