The representatives confirmed the split to Usmagazine.com.
Barker and Moakler, who have two children together, divorced in 2004, but reconciled last September after Barker was injured in a plane crash that left four dead.
A source told Usmagazine.com Wednesday the reunion was short-lived, however, and the pair broke up "after a nasty fight broke out at his house last night and cops were called ... (when) Shanna started freaking out and acting irrational."
No charges were filed in the incident, which the source said started after Barker confronted Moakler about allegedly having an affair with actor Gerard Butler while Barker was in the hospital following the crash, the magazine's Web site said.
"After surviving the plane crash, his driving force in life is the love he has for his kids. He is tired of all other drama and is ready to move on," Barker's publicist said.
"They did break up but Shanna never cheated on Travis while he was in the hospital. Furthermore, Shanna is tired of playing out their personal relationship in the press," Moakler's representative added.