The game-worn jersey from Harper's first major league opening day sold for just over $13,000. He hit both of game's home runs in the win against the Miami Marlins.
Harper took to Twitter to express his disappointment.
The MLB auction site says that proceeds from the sale are given to charity.
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Washington Nationals slugger Bryce Harper was the last to know his opening-day jersey was up for auction, according to his Twitter feed.
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