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The zoo said the pup is the first red wolf born at the zoo since 2005.
"The zoo's zookeepers and veterinary team continue to monitor mom and baby through the use of an infrared camera located inside the wolves' newly built birthing den," the zoo said in the birth announcement. "While the pup has been observed nursing and appears to steadily gain weight, the next month is a critical time for the pup's development."
Red wolves are considered to be the world's most endangered canid species, with 15-20 remaining in the wild.