The clip, released Thursday, begins with Timberlake leading an unknown partner through an outdoor adventure that includes crossing a tree branch and riding in a boat before they arrive at a dance hall cabin.
As Timberlake brings his partner onto the dance floor, Biel appears in frame and the pair dance together under a number of spotlights.
"I brag about you to anyone outside, but I'm a man of the woods it's my pride," Timberlake sings on the track, which shares the same name as his new album.
The "Man of the Woods" video follows three other music videos the singer has released for songs "Say Something," "Supplies" and "Flighty."
Timberlake will be taking the stage during the halftime show of Super Bowl LII on Sunday.