Orly Adelson, president of Dick Clark Productions, made the announcement Monday.
The performance marks the first time both groups will perform on the same stage together for a national televised audience prior to their summer 2011 tour.
"New Kids on the Block and Backstreet Boys on one stage is something special. Moments like these are what the AMAs are all about," AMA producer Larry Klein said in a statement.
The boy bands join the previously announced line-up of Rihanna, Diddy-Dirty Money, Ne-Yo, Justin Bieber, Katy Perry, Ke$ha, Christina Aguilera, Bon Jovi, P!nk and Usher.
The show is to be broadcast live from the NOKIA Theatre L.A. LIVE Nov. 21 on ABC.