Karen Valentine (born May 25, 1947; Sebastopol, California) is an American actress of Italian and Portuguese extraction. She is best-known for her role as the idealistic schoolteacher "Alice Johnson" in the television series Room 222.

Valentine grew up on a Northern Californian chicken ranch. She won a spot representing California at the Miss Teenage America pageant where she was awarded the Talent Award. When Ed Sullivan saw her act (where she would comically dance in a flamboyant costume while lip-syncing to Eydie Gormé's "Blame it on the Bossa Nova"), he was quoted as saying, "I want that girl on my show." Valentine also was Miss Sonoma County and Top 10 at Miss California, state finals to Miss America.

In 1969, Valentine won her breakthrough role as a new teacher on the ABC television series Room 222, with Lloyd Haynes and Michael Constantine. She was nominated twice for an Emmy and once for a Golden Globe, winning an Emmy in 1970 for Outstanding Actress in a Supporting Role.

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