The partners are opening popup vaccination sites in the United States' two largest cities in an effort to serve people from "under-vaccinated" neighborhoods.
The New York City site, launched Thursday, is located at St. John's Bread & Life, which helps poor in the city. The Los Angeles site will open next week.
Amazon and Carbon Health say they expect to give as many as 150 vaccinations per day in both cities through the summer.
"We are proud to extend our collaboration with Amazon Studios to reach under-vaccinated communities, like unhoused individuals served by Bread & Life," Carbon Health Chief Medical Officer Dr. Sujal Mandavia tweeted.
Officials at the New York site said they hoped the effort would give a "renewed sense of hope, safety and security."
In February, Carbon Health worked with Los Angeles officials to vaccinate thousands of people at Dodger Stadium.