Watkins on Friday was listed as out on the team's final injury report. The speedy wideout has been dealing with a calf injury that prevented him from practicing all week.
The Chiefs also listed their leading rusher, Clyde Edwards-Helaire, as questionable for Sunday's game against the Browns because of hip and ankle injuries. The rookie running back was a limited participant in Wednesday's practice, but he did no team work Thursday and Friday.
"A couple of days ago he moved around real well, and then we just kind of backed off of him," Chiefs head coach Andy Reid told reporters Friday. "He's still doing stuff, just not with the group. We'll just see how he does."
Watkins has missed six games this season due to injuries. He was fifth on the team with 37 catches, 421 yards and two touchdowns. Kansas City will likely replace him with Demarcus Robinson, who was third on the team with 45 receptions.
If Edwards-Helaire is unable to suit up, the Chiefs are expected to use a combination of Pro Bowl tailback Le'Veon Bell and Darrel Williams. The duo combined for 423 yards and three touchdowns for the Chiefs this season.