The Giants on Friday ruled out Beckham for Sunday's matchup at the Indianapolis Colts due to a quadriceps injury that has sidelined him for the past two games.
Beckham was unable to practice all week for New York, which officially was eliminated from postseason contention with last week's 17-0 home loss to the Tennessee Titans.
Earlier this week, Giants coach Pat Shurmur dismissed the idea of shutting down Beckham for the season because the team had nothing to play for. Beckham told reporters on Thursday that he planned to play if he was healthy.
Beckham initially was hurt in a 25-22 loss at Philadelphia on Nov. 25, but played the following week against the Chicago Bears, posting three catches for 35 yards and a touchdown in a 30-27 victory.
Although he practiced during the week, Beckham reported discomfort in the quad a week ago and the Giants announced that he would not accompany the team to Washington in Week 14.
Beckham also was ruled out of last week's game after undergoing valuation at the Hospital for Special Surgery in Manhattan. Beckham said that the tests were conducted to double-check the injury and ensure that he was healing.
The injury was described as being consistent with a hematoma, where a mass of blood pools in one area, according to the New York Daily News.
Limited to four games due to a fractured ankle in 2017, Beckham has 77 catches for 1,052 yards and six touchdowns this season. He signed a five-year contract extension worth $95 million, including $65 million guaranteed, in late August.
A first-round pick (No. 12 overall) in the 2014 NFL Draft, Beckham had at least 90 catches and 1,300 yards in each of his first three seasons with New York. He also had double-digit touchdowns in each of those seasons, including a career-best 13 in 2015.
Beckham reached 200 receptions and 3,000 receiving yards in just 30 games, getting to those milestones faster than anyone in NFL history.