Freeman, 22, had 13 carries for 37 yards and a touchdown in the victory. He suffered the ankle injury halfway through the third quarter.
The third-round pick in the 2018 NFL Draft out of Oregon has 71 carries for 309 yards and four touchdowns on the ground in seven games this season.
Fellow rookie Phillip Lindsay had 14 carries for 90 yards and a score in Thursday's blowout. He has 436 yards and two scores on the ground this season, while averaging an impressive 5.8 yards per carry.
The Broncos own the No. 10 rushing offense in the NFL, averaging 125.1 yards per game. Denver faces the Chiefs in an AFC West showdown at 1 p.m. on Oct. 28 at Arrowhead Stadium in Kansas City.