The team announced in a statement on Thursday night that McCloughan was fired after just two years on the job.
"The Washington Redskins have released Scot McCloughan from the organization effective immediately," the team said in the statement. "We wish him success in his future endeavors. The team will have no further comment on his departure.
"The organization remains confident in our personnel department as we execute our free agency plans as well as prepare for the upcoming NFL Draft."
Reports indicated that the move was related to ongoing issues with alcohol. A drinking problem led to his firing from previous front-office positions with the San Francisco 49ers in 2010 and the Seattle Seahawks in 2014, The Washington Post reported.
"He's had multiple relapses due to alcohol," an anonymous source told The Post. "He showed up in the locker room drunk on multiple occasions. ... This has been a disaster for 18 months."
The move was speculated for several weeks after reports surfaced that McCloughan was cut out of the Redskins' decision-making process. He was not involved in coach Jay Gruden's contract extension, the re-signing of tight end Vernon Davis or the exclusive tag placed on quarterback Kirk Cousins.
The 46-year-old McCloughan also did not attend the NFL Combine last week in Indianapolis and was not seen at Redskins Park leading up the start of the free agency signing period. He said he missed the combine because of the death of his grandmother on Feb. 6.
McCloughan's staff went through interviews with college players without him at the Combine. The Redskins have 10 picks in this year's draft.