But Thomas wasn't the only athlete or entertainer on the other end of a challenge from the "Black Mamba."
His commercial for Nike Basketball was 100 percent "Mamba" motivation
"Are you made for the Mamba Mentality?" Bryant asked in the video.
"Do you learn from your mistakes? Can you draw strength from your failures? Can you block out everything and be completely in the moment?"
"Do you check your fear at the door? Are you willing to suffer for your dreams? That's the Mamba Mentality. The continual quest to be great. So if you think you have what it takes, it's time to get started."
Bryant reposted the video and issued challenges in the replies.
"@Isaiah_Thomas I challenge you to make the All-NBA First Team next season #MambaMentality," Bryant wrote.
He also challenged Toronto Raptors guard DeMar DeRozam.
"@DeMar_DeRozan I challenge you to rekindle a lost friendship from your youth in Compton #MambaMentality," he tweeted.
Bryant also issued a request to rapper Kendrick Lamar.
"@KendrickLamar I challenge your record label to revolutionize the music program at Centennial High School #MambaMentality," he tweeted.
"@allysonfelix I challenge you to coach the sprinters for the upcoming Special Olympics #MambaMentality," Bryant wrote to the United States Olympic track star.
"@RSherman_25 I challenge you to break the Seahawks single season interception record #MambaMentality," Bryant wrote.
Sherman, 29, had four interceptions last season. He had a career-high eight picks in 2012 and 2013. He would have to intercept 11 passes this season to break the franchise record for a single season.
The commerical spot was for Bryant's new Kobe AD sneakers, which were released Thursday.