The association called Machado's suspension a "slap in the face" Tuesday. Machado was handed the suspension for making contact with an umpire during the Padres' Saturday game against the Colorado Rockies.
Machado was ejected for arguing a called third strike, at one point appearing to make contact with plate umpire Bill Welke.
The umpires association said on Twitter on Tuesday that Machado getting just a one-game suspension is "a disgrace to the game."
Machado is appealing the one-game suspension and can play until the appeal is heard.
"I didn't think I touched him," Machado said Monday. "The video says it all. We're going to appeal it, and we think we've got a good case."
This season Machado is hitting .261 with 13 home runs and 39 runs batted in.