The Blue Jays also sent right-handed pitcher Chris Smith to Buffalo on option and activated right-hander Mike Bolsinger from the 10-day disabled list, where he had been with left knee inflammation.
Tulowitzki fell after stepping on first base as he tried to beat out a grounder during the third inning of the 7-2 loss to the Los Angeles Angels on Friday night. He was helped off the field by two trainers.
Tulowitzki is batting .249/.300/.378 with seven home runs and 26 RBIs in 66 games this season. He also was on the DL April 22-May 25 with a strained hamstring.
Refsnyder was acquired last Sunday from the New York Yankees in exchange for first baseman Ryan McBroom, who was with Double-A New Hampshire.
In four games at Buffalo, Refsnyder was hitting .417 (5-for-12) with two RBIs and three walks. In 20 games with the Yankees this season, he batted .135 (5-for-37) with a double and a triple.
Smith has a 5.40 ERA in four appearances with Toronto.