The 33-year-old left-hander threw one pitch in the second inning before drawing a mound visit from Dodgers manager Dave Roberts and a trainer. He was immediately yanked from the game.
He was scheduled to be re-evaluated Tuesday morning.
"It was during (pregame) warm-ups where everything started tightening up on me, pretty much every part of the hip," Kazmir said via ESPN.com. "I couldn't fire my back hip, my back leg and there was no power or direction. That's a tough way to pitch."
Kazmir allowed one hit and struck out one in his one-plus inning Monday. Colorado went on to beat Los Angeles 10-9.
In his only other spring start, Kazmir gave up two runs (one earned) on one hit and two walks with one strikeout in 1 2/3 innings on March 1 against the San Francisco Giants.
The three-time All-Star signed a three-year, $48 million deal with the Dodgers before the 2016 season. He posted a 10-6 record with a 4.56 ERA and 134 strikeouts last year.