That leaves the Eagles without their best offensive lineman untile Monday, Dec. 19.
"We're very disappointed that Lane Johnson has been suspended 10 games. His actions have affected the entire organization," Eagles general manager Howie Roseman said. "We are all working under a clearly-defined set of rules and regulations set forth by the NFL in the collective bargaining agreement with the NFLPA. Moving forward, he has no choice but to hold himself accountable to Jeffrey Lurie, Coach Pederson and his staff, his teammates, and to the entire organization and our fans. When he returns from suspension, we will continue to support him and welcome him back to the team. We are moving on with our preparations for this week's game in Washington."
Johnson had an appeals hearing with the league in which he said he spent nearly eight hours outlining his reasons for overturning his latest violation of league policy.
The 26-year-old was the fourth overall pick in the 2013 draft and said he failed a test for performance-enhancing drugs after taking a supplement with peptides. Johnson said peptides were not listed among active ingredients.
During an appeal described by Johnson as "eight hours of bickering," he said he allowed his attorneys to do all the talking.
The appeal focused on a flaw in the screening system, according to NFL.com, citing Johnson's attorneys.
Johnson was suspended four games for a PED violation in 2014. He signed a six-year, $66.1 million contract in January.