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A Guinness World Records adjudicator was in the audience and confirmed after the performance that the group broke the record for the oldest choir.
The singers were all residents from seven care homes run by Runwood Homes, which is based in Stratford-Upon-Avon.
The concert at Crowne Plaza Hotel's Grand Ballroom also featured an appearance by singer Tony Christie, who joined the choir for performances of "Amarillo" and "Silent Night."