Pearl's mother's sister, Miracle Milly, previously held the same record at a height of 3.8 inches. Milly died in 2020, before Pearl was born.
"We're blessed to have her," Semler told Guinness World Records. "And to have this unique opportunity to break our own record and share with the world this amazing news."
The shortest dog ever, a dwarf Yorkshire terrier owned by British man Arthur Marples, was measured at 2.8 inches tall before his death in 1945.