"Sometimes your patient just needs you to go the extra mile to make them feel comfortable when they're here for the day to have surgery," the animal hospital wrote. "Dr. Ross has mastered the technique and sweet Ruby was so thankful for that."
The video, which has been viewed more than 460,000 times, features Henderson performing the song while the 6-month-old puppy rests peacefully on his leg.
Henderson, 28, told ABC News he has been singing to animals at the hospital for several months to help make their stay more pleasant.
"Dogs spend 99 percent of their time at home and when they come into the hospital, we try to give them as pleasant an experience as possible," he said. "I think that's why attention and music, those two pieced together, causes a little bit of familiarity to them. It's the best part when they start to settle down and say, 'OK, I can relax now.'"
Fellow veterinarian Darcy Holloway, who captured the viral video, said Henderson's song quickly changed the puppy's mood.
"Ruby was back there jumping on the cage and she was crying," Holloway said. "We don't know if it's the one-on-one attention or if it's the song, but they usually respond really well to it."