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The video, captured April 5 but just released to the public Tuesday, shows three pups roughhousing under the watchful eyes of their mother and father.
"Not only is it the first natal den of the animal ever discovered in the region," Nelson said. "It's the first time that pups have been recorded on video."
Nelson said the identity of the foxes was confirmed by a fur sample sent to the University of California-Davis canine genetics lab.
The wildlife camera is one of 16 set up in the Deschutes National Forest with assistance from the Oregon Zoo Foundation's Future for Wildlife program.