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Investigations suggest a large aqueduct pipe broke releasing water that eroded the part of the road, causing the sinkhole which swallowed about 20 cars.
"This is almost certainly a phenomenon related to water infrastructure," Alessandra Biserna, of the National Geology Advisory said according to The Local.
No injuries were reported at the scene although the city's mayor, Dario Nardella, described the incident as "very serious."
Emergency services also arrived at the scene as they attempted to prevent any further damage to the surrounding area.
"The measures that are being taken right now are necessary," Biserna said of the decision to close off the area. "Also because of the potential impact on the surrounding infrastructure."