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The droppings are displayed in illuminated resin spheres after being preserved in a specially-designed drying machine.
"Poo is all around us and inside us, but we ignore it," co-curator Daniel Roberts told the BBC.
Another co-curator, Nigel George, said poo "provokes strong reactions" in people.
"Small children naturally delight in it but later we learn to avoid this yucky, disease-carrying stuff, and that even talking about poo is bad," he said. "But for most of us, under the layers of disgust and taboo, we're still fascinated by it."
The museum is planned to remain on the Isle of Wight through the end of the summer before going on tour.