After passing the driving test, the 16-year-old was told the picture could not be taken "because he did not look the way a boy should."
"They said he was wearing a disguise," Culpepper's mother, Teresa Culpepper, told WYFF News 4. "It was very hurtful. He was absolutely devastated. That's who he is 24/7."
According to the DMV, employees were just following the rules.
Culpepper did end up taking off the makeup and getting the picture taken.
The Transgender Legal Defense and Education Fund put out a release calling for the SCDMV to allow Culpepper to retake the picture.
"Chase's freedom to express his gender should not be restricted by DMV staff," said TLDEF Executive Director Michael Silverman. "He is entitled to be who he is and to express that without interference from government actors."
According to the TLDEF, forcing Culpepper to take off his makeup is a violation of his right to free speech.
"This is who I am and my clothing and makeup reflect that," Culpepper said. "The Department of Motor Vehicles should not have forced me to remove my makeup simply because my appearance does not meet their expectations of what a boy should look like. I just want the freedom to be who I am without the DMV telling me that I'm somehow not good enough."