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The study, which deducted manliness points from cities with large numbers of cupcake stores or fancy furniture sellers, ranked Nashville in the top spot, followed by Charlotte, N.C.; Oklahoma City; Memphis and Columbia, S.C.
Los Angeles ranked last on the list with San Diego ranking No. 49.
The Board of Manliness, which helped rank the cities, included actor Lou Ferrigno, baseball Hall of Famer Rollie Fingers, retired wrestler Sergeant Slaughter, comedian Judy Gold and champion lumberjack Mike Sullivan.