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"An inquiry found evidence of liturgical and financial irregularities as well as lifestyles that were probably not in keeping with that of a monk," Benedettini said. "The church remains open but the monks are awaiting transfer."
Benedettini said the move is part of Pope Benedict XVI's crackdown on "loose living" in the Catholic Church.
The basilica of Santa Croce in Gerusalemme houses some of the Catholic Church's holiest relics, including nails and wood splinters said to be from Jesus' cross and thorns from his crown.