The zombie drama will see the two former enemies "traveling into a post-apocalyptic Manhattan, long ago cut off from the mainland," the network said in a synopsis Saturday.
"The crumbling city is filled with the dead and denizens who have made New York City their own world full of anarchy, danger, beauty, and terror."
A brief teaser clip sees Maggie enlisting Negan's help to rescue her son Hershel, who has been kidnapped.
Negan infamously killed Maggie's husband Glenn (Steven Yeun) when Maggie was pregnant with Hershel.
The Walking Dead -- which took place largely in the American South -- wrapped its 11-season run in November.