The live audience will consist of 20 fans, 60 percent of normal capacity inside the WWHL studio, known as The Clubhouse.
The June 13 episode will also feature Cohen's first live guests since the pandemic began with Uzo Aduba and John Benjamin Hickey joining the show.
"I can't wait to welcome back my gang of 20 cocktail companions. Also -- throwing a party for one every night was starting to get extremely depressing," Cohen said in a statement.
Cohen started to host WWHL from his home in March 2020. He returned to the Clubhouse in October.
Other talk shows have also started allowing live audiences back in, including The Tonight Show Starring Jimmy Fallon. The Late Show with Stephen Colbert will return to Ed Sullivan Theater in New York on June 14 with a full, vaccinated audience.