The two-hour special will help benefit nonprofit organization Broadway Cares/Equity Fights AIDS, which provides supplies and financial assistance to those in the Broadway community who are struggling during the COVID-19 pandemic.
Sutton Foster (The Music Man), Brittney Mack (Six: The Musical), Rob McClure (Mrs. Doubtfire: A New Musical) and Mary-Louise Parker (How I Learned to Drive) have also joined the special, which will be presented with reduced commercials by presenting partner T-Mobile.
The show will provide sneak peaks at Broadway shows that will launch next year.
Tina Fey is serving as the host. The casts of Fey's Mean Girls play, Jersey Boys, Rent, Jagged Little Pill, Chicago, Ain't Too Proud -- The Life and Times of the Temptations and Diana: The Musical will be performing, along with Kelly Clarkson, Patti LeBelle and Brett Eldredge.
The cast of Harry Potter and the Cursed Child, Billy Porter, Kristen Bell, Jake Gyllenhaal, Alanis Morissette, Annaleigh Ashford, Lance Bass, Ron Cephas Jones, Jesse Tyler Ferguson, Peter Gallagher, Josh Groban, Sean Hayes, Nathan Lane, Camryn Manheim, Jerry O'Connell, Leslie Odom Jr., Jon Stamos, Aaron Tveit, Blair Underwood, Vanessa Williams and Susan Kelechi Watson will also be making appearances.
Streisand honored essential workers amid the COVID-19 pandemic in May with a tribute music video for her recording of "You'll Never Walk Alone," a song from musical Carousel.