To be taped in Sydney, the six-episode show will feature 10 Australian comedians battling for the top prize of $68,000.
Set to debut in 2020, it follows hit versions from Japan and Mexico that are already streaming.
"I'm thrilled to be joining the Prime Video family of creators and talent," Wilson said in a press release. "I've already had the chance to work with Alexa -- she's great -- and on Audible, so working with Prime Video seemed like an obvious choice. I can't wait to see what these talented Australian comedians come up with and how far they'll go to take home the prize."
Wilson is known for her work in the films Bridesmaids, Night at the Museum: Secret of the Tomb and the Pitch Perfect franchise.
She will soon be seen in the films Cats and Jojo Rabbit.