"You sure you wanna do this?" Corden asked the actor confidently before he began his rap. "C'mon man I ain't scared of you, let's go," Jackson replied.
To win the contest, the late night host attacked the actor's successful film career and fashion sense. "It's another Drop the Mic where I end up an abuser to prove the 'L' in Samuel L. Jackson stands for loser," Corden rapped before noting, "He's the highest-grossing actor, that's a fact. That's how he buys all those damn Kangol hats."
In response, Jackson poked fun at Corden's celebrity status and popular Carpool Karaoke segments. "I feel bad for you son, 'cause I'm the real star. No one knows who are you are without Adele in a car," he said before flipping Corden the bird after rapping, "In the Emoji Movie I hear you just play a hand, so look, I'm going to put it in a way you can understand."
Other standout rhymes included Corden bringing up Jackson's roles in Jurassic Park and Pulp Fiction and Jackson referencing his infamous line from Snakes on a Plane.
"You got your [expletive] kicked by a man twice your age. Now get this mother [expletive] hack off this mother [expletive] stage!" Jackson quipped before the audience declared him the winner.
The 68-year-old was last featured on The Late Late Show in March when he paid homage to some of his most iconic roles alongside Corden.
Jackson will next be seen opposite Ryan Reynolds in The Hitman's Bodyguard which arrives in theaters Friday.