The ensemble for the three-part miniseries -- which was written by Heidi Thomas -- will also include Emily Watson, Willa Fitzgerald, Annes Elwy, Kathryn Newton and Jonah Hauer-King.
Director Vanessa Caswill is scheduled to start principal photography on the project this month in Ireland. The BBC One series is a collaboration with the Playground production company and Masterpiece on PBS.
"Loved by generations worldwide, Little Women is a truly universal coming-of-age story, as relevant and engaging today as it was when originally published in 1868," a press release noted. "Set against the backdrop of a country divided, the story follows the four sisters Meg, Jo, Beth and Amy March on their journey from childhood to adulthood. With the help of their mother Marmee, while their father is away at war the girls navigate what it means to be a young woman -- from gender roles to sibling rivalry, first love, loss and marriage."
Lansbury will play the girls' wealthy Aunt March, while Gambon will portray their benevolent neighbor Mr. Laurence.