Nelson, Alison Krauss, the Avett Brothers, Jack Johnson, Kris Kristofferson, Lee Ann Womack, Lyle Lovett, Norah Jones and the Little Willies, Sheryl Crow, Susan Tedeschi & Derek Trucks and Vince Gill are also set to take the stage on Jan. 12 at the Bridgestone Arena in Nashville.
More performers will be announced at a later date.
The concert, titled Willie: Life & Songs of an American Outlaw, will feature the lineup performing some of Nelson's greatest hits. The event will be filmed for a television special that will be aired on A&E in 2019.
Tickets go on sale for the general public on Nov. 12 at 10 a.m. CT through LiveNation.
Nelson released his 68th studio album My Way, in September. The album was a tribute to Frank Sinatra and followed the release of Last Man Standing in April.