The 39-year-old rapper called out the 26-year-old singer at Drake's concert Wednesday after wife Kim Kardashian shared a secretly recorded conversation of West and Swift discussing the song.
"I am so glad my wife has Snapchat. 'Cause now y'all can know the truth. And can't nobody talk [expletive] about Ye no more!" he told the audience before performing the controversial track.
Kardashian released the conversation this month on Snapchat after telling GQ that Swift had approved the song's lyrics. The track includes the line, "I feel like me and Taylor Swift might still have sex. Why? I made that [expletive] famous."
"I feel like I've had it with people blatantly treating my husband a certain way," she said of the GQ interview on Keeping Up with the Kardashians. "At this point, I don't really give a [expletive], so I'll do whatever to protect my husband."
The Snapchat clips see Swift give West permission to use her name in the song. The singer has denied approving the "that [expletive]" lyric and accused the rapper and Kardashian of "falsely" painting her as a liar.
"He promised to play the song for me, but he never did," she said. "While I wanted to be supportive of Kanye on the phone call, you cannot 'approve' a song you haven't heard."
West and Swift's feud began in 2009 when the rapper interrupted Swift's acceptance speech at the MTV Video Music Awards. "Famous" is nominated for Video of the Year and Best Male Video at this year's ceremony, which airs Aug. 28.