The clip, released Tuesday, features Emmet meeting the daring and adventurous Rex Dangervest --also voiced by Pratt -- who pays homage to the actor's roles in the Jurassic World series as he is also a raptor trainer.
Emmet joins up with Rex to find Lucy inside the Sis-Star System who finds herself meeting with ruler Queen Watevra Wa'Nabi, voiced by Tiffany Haddish.
Lego Batman (Will Arnett) also makes an appearance as Emmet and his friends battle new Lego Duplo invaders.
The Lego Movie 2: The Second Part will arrive in theaters on Feb. 8. Original directors Phil Lord and Chris Miller are producing the sequel with Trolls helmer Mike Mitchell directing from a script by BoJack Horseman creator Raphael Bob-Waksberg and Mike Fogel.
Nick Offerman, Alison Brie, Stephanie Beatriz, and Arturo Castro also provide voice work.