The clip, released Monday, focuses more on the film's central villains as Jordan plots to take over Wakanda as Erik Killmonger and Serkis as Ulysses Klaue escapes from an interrogation.
"A war is coming," a voice says to Boseman who replies, "Let them come."
The trailer also includes an extended look at an action-packed car chase scene in the film where Black Panther is seen leaping across a highway.
The end of the footage announces that pre-sale tickets for Black Panther are on sale through Fandango.
Directed by Ryan Coogler (Creed), Black Panther is set to release in theaters on Feb. 16. The film also stars Lupita Nyong'o, Angela Bassett, Martin Freeman, Forest Whitaker and Dani Gurira among others.