The action-packed clip, released Monday, features Boseman leaping into action as the Black Panther as he protects his home country and the world from the villainous Erik Killmonger (Michael B. Jordan).
"The Black Panther has protected the world for generations," Boseman says. "It is your time," Bassett, who portrays the hero's stepmother, Ramonda, adds.
The trailer also includes glimpses of Andy Serkis as villain Ulysses Klaue and Lupita Nyong'o as Dora Milaje member Nakia. In the pages of Marvel comics, the Dora Milaje were an elite group of female bodyguards who protect the king of Wakanda.
Directed by Ryan Coogler (Creed), Black Panther is set to release in theaters on Feb. 16.
Boseman will also be seen as Black Panther in Avengers: Infinity War, which releases on May 4. A trailer for the ensemble film featured the actor teaming up with the likes of Captain America (Chris Evans), Winter Soldier (Sebastian Stan) and others to defend Wakanda from the mighty Thanos (Josh Brolin).