The clip, released Tuesday, features Del Toro and Brolin reprising their roles as undercover operative Alejandro Gillick and CIA agent Matt Graver, respectively, as they rejoin the war against drugs.
The sequel will explore how drug cartels have started smuggling terrorists into the United States from Mexico and how Del Toro and Brolin plan on stopping it.
"Turning you loose," Brolin says to Del Toro. "No rules this time."
Matthew Modine, Catherine Keener, Manuel Garcia-Rulfo, and Jeffrey Donovan also star. Original star Emily Blunt is not returning. Director Stefano Sollima takes over for previous helmer Denis Villeneuve with the script again being handled by Taylor Sheridan.
Sicario 2: Soldado is set to arrive in theaters on June 29.