The 35-year-old singer and 34-year-old actress attended the 2016 Critics' Choice Awards at Barker Hangar in Santa Monica, Calif., where Timberlake's movie Trolls was nominated for two awards.
Timberlake and Biel were all smiles as they posed together for photos. The singer looked dapper in a black suit, while Biel stunned in a striped Elie Saab dress with a plunging neckline and lace inset sleeves.
Trolls was up for Best Animated Feature and Best Song for "Can't Stop the Feeling," but lost to Zootopia and La La Land for "City of Stars," respectively. Timberlake jokingly poked fun at "Can't Stop the Feeling" while presenting Best Acting Ensemble.
"Turn that song off!" he said when the tune accompanied his entrance on stage. "Was that out loud?"
Arrival star Amy Adams, La La Land star Emma Stone and Lion star Nicole Kidman were among the other celebrities at the event. La La Land was the big winner of the night, taking home eight awards, including Best Picture.
Timberlake and Biel married in Fasano, Italy, in 2012, and welcomed their first child, son Silas, in April 2015. The family dressed up as Trolls characters for Halloween, and Timberlake later told TheFix that Silas recognizes "Can't Stop the Feeling."
"He knows that ['Can't Stop the Feeling'] is my song," he revealed. "We just say, 'That's daddy's song, that I wrote the song."