Harry and Markle will move from Kensington Palace to Frogmore Cottage on the Windsor grounds. The couple's new office will be based out of Buckingham Palace.
"This long-planned move will ensure that permanent support arrangements for The Duke and Duchess's work are in place as they start their family and move to their official residence at Frogmore Cottage," the palace said.
Harry and Markle will appoint new communications staff to their team. The couple have named Jason Knauf senior adviser and Christian Jones communications secretary, with Sara Latham to head up communications.
William, duke of Cambridge, and Middleton, duchess of Cambridge, will maintain their office at Kensington Palace. The couple live at the palace with their three children, Prince George, Princess Charlotte and Prince Louis.
Harry and Markle married in May and announced in October they are expecting their first child. The couple celebrated Commonwealth Day by attending a service at Westminster Abbey with the royal family this week.