Forte and Sudeikis have performed together on Saturday Night Live and on Last Man on Earth.
Halfway through the song, host Jimmy Fallon, another SNL alum, joins the duo and encourages the audience to sing along as well.
Forte currently stars on Last Man on Earth with Kristen Schaal and January Jones. Sudeikis joined the show in its second season.
Sudeikis most recently lent his voice to The Angry Birds Movie as Red.