The non-profit charity held its fourth televised fundraiser, broadcast without commercials on more than 30 networks and streamed on Yahoo and Hulu.
Remission is available to download through various digital retailers, royalties from which will be donated to the Stand Up to Cancer charity.
"I love Stand Up to Cancer," he said. "But we shouldn't stop there? As a society we need to stand up to Libras and Pisces and Tauruses and Capricorns"
Organizers don't yet know how much money was raised Friday night, but hoped to raise more than $100 million. The previous three telecast events raised $261 million combined.