Robert Hamilton (September 14, 1753 - March 8, 1809) was a businessman, judge and political figure in Upper Canada.

He was born in Bolton, East Lothian, Scotland in 1753. He came to Canada as part of a contract with a fur trading company operating west of the Great Lakes. In 1780, he formed a partnership with Richard Cartwright to supply goods to the British army at Fort Niagara which later expanded to include the transportation of goods across the province. Around 1784, he settled at Queenston. In 1791, with others, he won the contract for transporting military goods through the portage at Niagara. In 1788, he was named to the land board of the Nassau District and a judge of the Court of Common Pleas. He opened a general store at Queenston and also acquired extensive land holding on the Niagara peninsula with his profits.

In 1792, he became a member of the Legislative Council for the province.