Nicole Holofcener (born March 22, 1960) is a film and television director.

Born in New York City, Holofcener grew up around show business with her actor father Lawrence Holofcener and set decorator mother Carol. As a child she once broke both her arms at once, when she was knocked off her bike by a car. With the help of her stepfather, film producer Charles H Joffe, she started as an assistant to editor Susan E. Morse, a frequent collaborator of Woody Allen. After studying film at Columbia University, she made a short titled Angry in 1991. She also briefly appeared in the film Mi Vida Loca.

Holofcener made her feature film writing and directing debut with the semi-autobiographical Walking and Talking, which starred Catherine Keener and Anne Heche. The film was critically acclaimed and has since gained a cult following. Her understanding of modern, professional women made her an ideal choice to direct female-centric television shows like Sex and the City, Leap of Faith and Gilmore Girls.