Infobox last updated on: May 25, 2009.

Klára Zakopalová (born as Klára Koukalová on February 24, 1982 in Prague, Czechoslovakia) is a Czech professional female tennis player. She was born and still lives in Prague, the capital.

In her career Zakopalová has reached eight singles finals and won two, in 2005 on the grass of Rosmalen (Netherlands) and in September of that year on the hard courts of Portorož (Slovenia).