Jo Anne Worley (born September 6, 1937) is an American actress. Her work covers television, movies, theater, game shows, talk shows, commercials, and cartoons. She is best known for her work on the comedy-variety show Rowan & Martin's Laugh-In.

Worley was born in Lowell, Indiana, the third of five children. In 1962, her father remarried and his second union gave her two half-brothers and two half-sisters. Always remembered for her loud voice, Worley once said that when she attended church as a little girl, she never sang the hymns but would only lip-synch them for fear that she would drown out everyone else. Before graduating from high school, she was named School Comedienne.

After graduating from high school in 1955, Worley moved to Blauvelt, New York, where she began her professional career as a member of the Pickwick Players. This led to a drama scholarship to Midwestern State University in Wichita Falls, Texas.