Jesse Crain (born July 5, 1981, in Toronto, Ontario, Canada) is a relief pitcher in Major League Baseball. He currently plays for the Minnesota Twins.

Crain attended Fairview High School in Boulder, Colorado. where he played baseball, basketball, and football. He was named 1999 All-State and Colorado High School Player of the Year. He was a 2001 graduate of San Jacinto Junior College in Texas, where he was an All-Conference shortstop. In 2002, he was transferred to the University of Houston and was named an all-conference shortstop and relief pitcher. He was named first team All-America by Baseball America and Baseball Weekly and second team All-America by the ABCA. He was also named to Conference USA All-Academic team.

Crain was drafted and signed in 2002, playing at the rookie level, and low-A minor league levels going 3-2 (wins/losses) with an 0.99 Earned Run Average combined. In 2003, he managed to pitch at all three levels in the minor leagues, going 6-3 with a 3.00 ERA. In 2004 he pitched with the AAA-ball Rochester Red Wings and went 3-2 with a 2.49 ERA before being called up by the Minnesota Twins.