The 60-year-old media mogul snapped her first-ever selfie at the 45th annual NAACP Image Awards in Pasadena, Calif., on Saturday.
"Did I just photo bomb Oprah???" he wrote.
Winfrey was up for a best supporting actress award for The Butler, but lost to 12 Years a Slave's Lupita Nyong'o.
Handler slams Piers Morgan: 'You're a terrible interviewer'
Beautician charged with giving client fatal silicone butt injection