The former couple was spotted chatting at the CAA and Moet Pre-Golden Globes party at Soho House in West Hollywood on Friday.
"It sounded like tonight was the first night they spoke in a year," an eyewitness tells Us Weekly.
Seacrest, 39, and Hough, 25, reportedly shared a conversation in a back garden around midnight. The two ran into each other about an hour later and began a new conversation.
According to the source, the American Idol host even invited the Dancing With the Stars alum and her date for the night, Nina Dobrev, to an after party that would take place at his house.
The pair ended their two-year relationship in March 2013 citing their busy schedules as the reason for the split. Since then, Seacrest has been linked to model Shayna Terese Taylor, with whom he was seen vacationing in Uruguay in November.